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Anti-AMFR Antibody Picoband® Fluoro488 Conjugated

Product Specifications

Background

Autocrine motility factor receptor, isoform 2 is a protein that in humans is encoded by the AMFR gene. Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins.

Synonyms

E3 ubiquitin-protein ligase AMFR;6.3.2.-; Autocrine motility factor receptor; AMF receptor; RING finger protein 45; gp78; AMFR; RNF45

Gene Name

AMFR

Gene ID

267

UniProt

Q9UKV5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human AMFR recombinant protein (Position: E553-S643) . Human AMFR shares 89% amino acid (aa) sequence identity with mouse AMFR.

Clonality

Polyclonal

Tissue Specificity

Abundant in hepatoma cells and fetal cochlea, ovary, eye, heart, adrenal gland, liver and kidney. Low levels present in adult peripheral blood leukocytes and fetal brain, thymus, spleen, skeletal muscle, lung and tongue. .

Applications

Flow Cytometry

Field of Research

Cancer, Cell Biology, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ring Finger E3 Ligase, Tumor Biomarkers, Ubiquitin E3 Enzymes

Purification

Immunogen affinity purified.

Form

Liquid

Function

E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD) . The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor. .

References & Citations

1. Lee, Joon No; Zhang Xiangyu; Feramisco Jamison D; Gong Yi; Ye Jin (Nov 2008) . Unsaturated fatty acids inhibit proteasomal degradation of Insig-1 at a postubiquitination step. J. Biol. Chem. (United States) 283 (48) : 33772–83. 2. Watanabe H, Carmi P, Hogan V, Raz T, Silletti S, Nabi IR, Raz A (Aug 1991) . Purification of human tumor cell autocrine motility factor and molecular cloning of its receptor. J Biol Chem 266 (20) : 13442–8.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

72996 MW

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

E3 ubiquitin-protein ligase AMFR

Subcellular Location

Endoplasmic reticulum membrane ; Multi-pass membrane protein .

Protein Name

E3 ubiquitin-protein ligase AMFR

Isotype

Rabbit IgG

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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