Anti-AK2 Antibody Picoband® Fluoro488 Conjugated

Adenylate kinase 2 is an enzyme is encoded in humans by the AK2 gene. The AK2 protein is found in the intermembrane space of the mitochondrion. Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.

Adenylate kinase 2, mitochondrial ; AK 2 ;2.7.4.3 ; ATP-AMP transphosphorylase 2 ; ATP:AMP phosphotransferase ; Adenylate monophosphate kinase ; Adenylate kinase 2, mitochondrial, N-terminally processed ; AK2 ; ADK2

E. coli-derived human AK2 recombinant protein (Position: E161-I239) . Human AK2 shares 93.7% and 93% amino acid (aa) sequence identity with mouse and rat AK2, respectively.

Present in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level) . .

Apoptosis, Cancer, Cell Biology, Cell Death, Energy Metabolism, Energy Transfer Pathways, Intracellular, Kinases, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis. .

1. Bruns GA, Regina VM (1977) . Adenylate kinase 2, a mitochondrial enzyme. Biochem. Genet. 15 (5–6) : 477–86. 2. Köhler C, Gahm A, Noma T, et al. (1999) . Release of adenylate kinase 2 from the mitochondrial intermembrane space during apoptosis. FEBS Lett. 447 (1) : 10–2.