Anti-Abhd5 Antibody Picoband®

1-acylglycerol-3-phosphate O-acyltransferase ABHD5 is an enzyme that in humans is encoded by the ABHD5 gene. The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated withChanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

1-acylglycerol-3-phosphate O-acyltransferase ABHD5;2.3.1.51; Abhydrolase domain-containing protein 5; Lipid droplet-binding protein CGI-58; ABHD5; NCIE2; CGI-58

E. coli-derived human Abhd5 recombinant protein (Position: R169-D349) . Human Abhd5 shares 96.7% amino acid (aa) sequence identity with both mouse and rat Abhd5.

Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level) . .

Cancer, Cancer Metabolism, Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Obesity, Pathways and Processes, Redox Metabolism, Signal Transduction

Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis (PubMed:18606822) . May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2 (PubMed:16679289) . Involved in keratinocyte differentiation (PubMed:18832586) . Regulates lipid droplet fusion (By similarity) . .

1. Ghosh AK, Ramakrishnan G, Chandramohan C, Rajasekharan R (Sep 2008) . CGI-58, the causative gene for Chanarin-Dorfman syndrome, mediates acylation of lysophosphatidic acid. The Journal of Biological Chemistry 283 (36) : 24525–33. 2. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011) . A conditional knockout resource for the genome-wide study of mouse gene function. Nature 474 (7351) : 337–342.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Cytoplasm . Lipid droplet . Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA (By similarity) . .