Anti-ABCE1 Antibody Picoband®

Boster Bio Anti-ABCE1 Antibody Picoband® catalog # PB10020. Tested in Flow Cytometry, IP, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

ATP binding cassette E1 (ABCE1, also RNase L inhibitor) is an ATPase found in humans involved in viral assembly. It is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily. ABCE1 inhibits the action of ribonuclease L. Ribonuclease L normally binds to 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) and inhibits the interferon-regulated 2-5A/RNase L pathway, which is used by viruses. ABCE1 heterodimerize with ribonuclease L and prevents its interaction with 2-5A, antagonizing the anti-viral properties of ribonuclease L, and allow the virus to synthesize viral proteins. It has also been implicated to have an effect in tumorcell proliferation and antiapoptosis.

Synonyms

ATP-binding cassette sub-family E member 1;2'-5'-oligoadenylate-binding protein; HuHP68; RNase L inhibitor; Ribonuclease 4 inhibitor; RNS4I; ABCE1; RLI, RNASEL1, RNASELI, RNS4I; OK/SW-cl.40

Gene Name

ABCE1

Gene ID

6059

UniProt

P61221

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human ABCE1 recombinant protein (Position: K419-D599) . Human ABCE1 shares 100% amino acid (aa) sequence identity with mouse ABCE1.

Clonality

Polyclonal

Tissue Specificity

Liver > pancreas > brain > kidney > heart > placenta.

Applications

Flow Cytometry, IP, IF, ICC, WB

Field of Research

Adapters, Antiviral Signaling, ATPases, Chaperones, Immune System Diseases, Immunology, Metabolism, Plasma Membrane, Protein Trafficking, Signal Transduction, Transmembrane

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly. .

References & Citations

1. Tian, Y; Han, X; Tian, DL (2012) . The biological regulation of ABCE1. IUBMB Life 64 (10) : 795–800.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

67314 MW

Observed Molecular Weight

67 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

Gene Name Synonym

ATP-binding cassette sub-family E member 1

Subcellular Location

Cytoplasm . Mitochondrion . Localized to clusters of virus formation at the plasma membrane.

Sequence Similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Protein Name