Anti-Visfatin/NAMPT Antibody Picoband® Fluoro488 Conjugated

Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme that in humans is encoded by the PBEF1 gene. This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.

Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt;2.4.2.12; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin; NAMPT; PBEF, PBEF1

E. coli-derived human Visfatin recombinant protein (Position: L62-N337) . Human Visfatin shares 97.1% and 96.7% amino acid (aa) sequence identity with mouse and rat Visfatin, respectively.

Adaptive Immunity, B Cells, Cancer, Cardiovascular, Cytokines, Growth Factors/Hormones, Hypoxia, Immunology, Innate Immunity, Metabolism, Metabolism Processes, Neurology Process, Neuroscience, Non-CD, Pathways and Processes, Serum Proteins, Signal Transduction

Catalyzes the condensation of nicotinamide with 5- phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity) . .

1. Entrez Gene: PBEF1 pre-B-cell colony enhancing factor 1. 2. Samal B, Sun Y, Stearns G, Xie C, Suggs S, McNiece I (February 1994) . Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor. Mol. Cell. Biol. 14 (2) : 1431–7. 3. Wang T, Zhang X, Bheda P, Revollo JR, Imai S, Wolberger C (July 2006) . Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme. Nat. Struct. Mol. Biol. 13 (7) : 661–2.

Nucleus . Cytoplasm . Secreted . Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum. .