Anti-Caldesmon/CALD1 Antibody Picoband® Fluoro550 Conjugated

Caldesmon is a protein that in humans is encoded by the CALD1 gene. It is mapped to 7q33. This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca (2+) -calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca (2+) -dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

E. coli-derived human Caldesmon recombinant protein (Position: M1-E120) . Human Caldesmon shares 87.6% amino acid (aa) sequence identity with rat Caldesmon.

High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments) . Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity) . .

1. Entrez Gene: CALD1 caldesmon 1. 2. Humphrey MB, Herrera-Sosa H, Gonzalez G, et al. (1992) . Cloning of cDNAs encoding human caldesmons. Gene 112 (2) : 197–204. 3. Novy RE, Lin JL, Lin JJ (Oct 1991) . Characterization of cDNA clones encoding a human fibroblast caldesmon isoform and analysis of caldesmon expression in normal and transformed cells. J Biol Chem 266 (25) : 16917–24.