Anti-Cofilin 2/CFL2 Antibody Picoband®

Boster Bio Anti-Cofilin 2/CFL2 Antibody catalog # RP1107. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Cofilin 2 (muscle), also known as CFL2, is a protein which in humans is encoded by the CFL2 gene. It is mapped to 14q12. This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. And this protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants.

Synonyms

Cofilin-2; Cofilin, muscle isoform; CFL2

Gene Name

CFL2

Gene ID

1073

UniProt

Q9Y281

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin 2, identical to the related mouse sequence.

Clonality

Polyclonal

Tissue Specificity

Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3 (PubMed:19752190) . It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere (By similarity) . .

References & Citations

1. Entrez Gene: CFL2 cofilin 2 (muscle) . 2. Agrawal PB, Greenleaf RS, Tomczak KK, Lehtokari VL, Wallgren-Pettersson C, Wallefeld W, Laing NG, Darras BT, Maciver SK, Dormitzer PR, Beggs AH (January 2007) . Nemaline myopathy with minicores caused by mutation of the CFL2 gene encoding the skeletal muscle actin-binding protein, cofilin-2. Am. J. Hum. Genet. 80 (1) : 162–7. 3. Gillett GT, Fox MF, Rowe PS, Casimir CM, Povey S (May 1996) . Mapping of human non-muscle type cofilin (CFL1) to chromosome 11q13 and muscle-type cofilin (CFL2) to chromosome 14. Ann. Hum. Genet. 60 (Pt 3) : 201–11.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

18737 MW

Observed Molecular Weight

26 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

Gene Name Synonym

Cofilin-2

Subcellular Location

Nucleus matrix . Cytoplasm, cytoskeleton . Colocalizes with CSPR3 in the Z line of sarcomeres. .

Protein Name