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Anti-Collagen I/COL1A1 Antibody Picoband®

Boster Bio Anti-Collagen I/COL1A1 Antibody Picoband® catalog # PB9939. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Pro-alpha1 chains

Synonyms

Collagen alpha-1 (I) chain; Alpha-1 type I collagen; Col1a1; Cola1

Gene Name

Col1a1

Gene ID

12842

UniProt

P11087

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I, different from the related human sequence by three amino acids, and identical to the related rat sequence.

Clonality

Polyclonal

Tissue Specificity

Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

Applications

IHC, WB

Field of Research

Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Type I collagen is a member of group I collagen (fibrillar forming collagen) .

References & Citations

1. Aihara, M., Lindsey, J. D., Weinreb, R. N. Ocular hypertension in mice with a targeted type I collagen mutation. Invest. Ophthal. Vis. Sci. 44: 1581-1585, 2003. 2. Cabral, W. A., Makareeva, E., Colige, A., Letocha, A. D., Ty, J. M., Yeowell, H. N., Pals, G., Leikin, S., Marini, J. C. Mutations near amino end of alpha-1 (I) collagen cause combined osteogenesis imperfecta/Ehlers-Danlos syndrome by interference with N-propeptide processing. J. Biol. Chem. 280: 19259-19269, 2005.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

138032 MW

Observed Molecular Weight

130,180-200 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Collagen alpha-1 (I) chain

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Protein Name

Collagen alpha-1 (I) chain

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.

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