Anti-TCP1 delta/CCT4 Antibody Picoband® Fluoro594 Conjugated

T-complex protein 1 subunit delta is a protein that in humans is encoded by the CCT4 gene. This gene is mapped to 2p15. The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha-transducin.

E. coli-derived human CCT4 recombinant protein (Position: R452-R539) . Human CCT4 shares 97.7% amino acid (aa) sequence identity with both mouse and rat CCT4.

Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta. .

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. .

1. Lee, M.-J., Stephenson, D. A., Groves, M. J., Sweeney, M. G., Davis, M. B., An, S.-F., Houlden, H., Salih, M. A. M., Timmerman, V., de Jonghe, P., Auer-Grumbach, M., Di Maria, E., Scaravilli, F., Wood, N. W., Reilly, M. M. Hereditary sensory neuropathy is caused by a mutation in the delta subunit of the cytosolic chaperonin-containing t-complex peptide-1 (Cct4) gene. Hum. Molec. Genet. 12: 1917-1925, 2003. 2. Won, K.-A., Schumacher, R. J., Farr, G. W., Horwich, A. L., Reed, S. I. Maturation of human cyclin E requires the function of eukaryotic chaperonin CCT. Molec. Cell. Biol. 18: 7584-7589, 1998.

Cytoplasm . Melanosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, cilium basal body . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .