What sizes are available for Anti-VRK1 Antibody Picoband® Fluoro647 Conjugated?

Anti-VRK1 Antibody Picoband® Fluoro647 Conjugated is available in the following size(s): 100 µg/Vial.

Is Anti-VRK1 Antibody Picoband® Fluoro647 Conjugated in stock?

Anti-VRK1 Antibody Picoband® Fluoro647 Conjugated is currently made to order / not held in immediate stock. Contact us for lead time.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Anti-VRK1 Antibody Picoband® Fluoro647 Conjugated

Q: What is the catalog number for Anti-VRK1 Antibody Picoband® Fluoro647 Conjugated?

The catalog number for Anti-VRK1 Antibody Picoband® Fluoro647 Conjugated is PB9907-Fluoro647.

Product Specifications

Background

Serine/threonine-protein kinase VRK1 is an enzyme that in humans is encoded by the VRK1 gene. This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. It is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.

Synonyms

Serine/threonine-protein kinase VRK1;2.7.11.1; Vaccinia-related kinase 1; VRK1

Gene Name

VRK1

Gene ID

7443

UniProt

Q99986

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human VRK1, different from the related mouse sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Highly expressed in fetal liver, testis and thymus. .

Applications

Flow Cytometry

Field of Research

Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics and Nuclear Signaling, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Purification

Immunogen affinity purified.

Form

Liquid

Function

Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr- 18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity. .

References & Citations

1. Entrez Gene: VRK1 vaccinia related kinase 1. 2. Nezu J, Oku A, Jones MH, Shimane M (Feb 1998) . Identification of two novel human putative serine/threonine kinases, VRK1 and VRK2, with structural similarity to vaccinia virus B1R kinase. Genomics 45 (2) : 327–31.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

45476 MW

Specificity

No cross reactivity with other proteins.

Applications Notes

Gene Name Synonym

Serine/threonine-protein kinase VRK1

Subcellular Location

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle . Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.

Protein Name

Serine/threonine-protein kinase VRK1

Isotype

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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