Anti-TRPM5 Antibody Picoband®

Transient receptor potential cation channel subfamily M member 5 (TRPM5) is a protein that in humans is encoded by the TRPM5 gene. This gene encodes a member of the transient receptor potential (TRP) protein family, which is a diverse group of proteins with structural features typical of ion channels. This protein plays an important role in taste transduction, and has characteristics of a calcium-activated, non-selective cation channel that carries Na+, K+, and Cs+ ions equally well, but not Ca (2+) ions. It is activated by lower concentrations of intracellular Ca (2+), and inhibited by higher concentrations. It is also a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. This gene is located within the Beckwith-Wiedemann syndrome critical region-1 on chromosome 11p15.5, and has been shown to be imprinted, with exclusive expression from the paternal allele.

Transient receptor potential cation channel subfamily M member 5; Long transient receptor potential channel 5; LTrpC-5; LTrpC5; MLSN1- and TRP-related gene 1 protein; TRPM5 ; LTRPC5 , MTR1

Strongly expressed in fetal brain, liver and kidney, and in adult prostate, testis, ovary, colon and peripheral blood leukocytes. Also expressed in a large proportion of Wilms' tumors and rhabdomyosarcomas. In monochromosomal cell lines shows exclusive paternal expression. .

Voltage-modulated Ca (2+) -activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent- specific, non-selective cation channel that mediates the transport of Na (+), K (+) and Cs (+) ions equally well. Activated ly by increases in intracellular Ca (2+), but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca (2+) . Also activated by a fast intracellular Ca (2+) increase in response to inositol 1,4,5- triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage- dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals. .

1. Emily R Liman (2010) . Changing Taste by Targeting the Ion Channel TRPM5.The Open Drug Discovery Journal 2: 98–102. 2. Liman ER (2007) . TRPM5 and taste transduction. Handb Exp Pharmacol. Handbook of Experimental Pharmacology 179 (179) : 287–98.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.