Anti-SKAP55/SKAP1 Antibody Picoband® Fluoro647 Conjugated
Product Specifications
Background
Src kinase-associated phosphoprotein 1 is an adapter protein that in humans is encoded by the SKAP1 gene. This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.
Synonyms
Src kinase-associated phosphoprotein 1; Src family-associated phosphoprotein 1; Src kinase-associated phosphoprotein of 55 kDa; SKAP-55; pp55; SKAP1; SCAP1, SKAP55
Gene Name
SKAP1
Gene ID
8631
UniProt
Q86WV1
Host
Rabbit
Reactivity
Human, Rat
Cross Reactivity
No cross-reactivity with other proteins.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SKAP55, different from the related mouse and rat sequences by two amino acids.
Clonality
Polyclonal
Tissue Specificity
Highly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level) . .
Applications
Flow Cytometry
Field of Research
Adapters, Cell Type Markers, Immunoglobulins, Immunology, Non-CD, Protein Phosphorylation, Receptors, Signal Transduction, Transmembrane, Tyrosine Kinases
Purification
Immunogen affinity purified.
Form
Liquid
Function
Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. .
References & Citations
1. Entrez Gene: SKAP1 src kinase associated phosphoprotein 1. 2. Marie-Cardine A, Bruyns E, Eckerskorn C, Kirchgessner H, Meuer SC, Schraven B (July 1997) . Molecular cloning of SKAP55, a novel protein that associates with the protein tyrosine kinase p59fyn in human T-lymphocytes. J Biol Chem 272 (26) : 16077–80. 3. Wang H, Moon EY, Azouz A, et al. (2003) . SKAP-55 regulates integrin adhesion and formation of T cell-APC conjugates. Nat. Immunol. 4 (4) : 366–74.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
41432 MW
Specificity
No cross reactivity with other proteins.
Applications Notes
6
Gene Name Synonym
Src kinase-associated phosphoprotein 1
Subcellular Location
Cytoplasm. Nucleus. Cell membrane. Upon T-cell stimulation, translocates to lipid rafts at the cell membrane.
Protein Name
Src kinase-associated phosphoprotein 1
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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