Anti-RAB7/RAB7A Antibody Picoband®

Ras-related protein Rab-7a is a protein that in humans is encoded by the RAB7A gene. RAB7A functions as a key regulator in endo-lysosomal trafficking, governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end ed endosomal migration and positions, and endosome-lysosome transport through different protein-protein interaction cascades. Furthermore, RAB7A is involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes through modulation of SOX10 and the oncogene MYC. Mutations in the lysosomal pathway result in tumor progression in melanoma cells.

A synthetic peptide corresponding to a sequence at the C-terminus of human RAB7, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

G Protein Signaling, Organelles, Protein Trafficking, Ras Family, Signal Transduction, Signaling Pathway, Small G Proteins, Subcellular Markers, Vesicle Transport

Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end ed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient- transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium) . In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed:11179213, PubMed:12944476, PubMed:14617358, PubMed:20028791, PubMed:21255211) . Regulates the endocytic trafficking of the EGF- EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (By similarity) . Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413) . .

1. Entrez Gene: RAB7A RAB7A, member RAS oncogene family. 2. Davies JP, Cotter PD, Ioannou YA (Apr 1997) . Cloning and mapping of human Rab7 and Rab9 cDNA sequences and identification of a Rab9 pseudogene. Genomics 41 (1) : 131–4. 3. Kashuba VI, Gizatullin RZ, Protopopov AI, Allikmets R, Korolev S, Li J, Boldog F, Tory K, Zabarovska V, Marcsek Z, Sumegi J, Klein G, Zabarovsky ER, Kisselev L (Dec 1997) . NotI linking/jumping clones of human chromosome 3: mapping of the TFRC, RAB7 and HAUSP genes to regions rearranged in leukemia and deleted in solid tumors. FEBS Letters 419 (2-3) : 181–5.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Cytoplasmic vesicle, phagosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Late endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Melanosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasmic vesicle, autophagosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Lipid droplet . Colocalizes with OSBPL1A at the late endosome (PubMed:16176980) . Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium (PubMed:21255211) . Lipid droplet localization is increased upon ADRB2 stimulation (By similarity) . .