Anti-Nestin Antibody Picoband®

Nestin  is a  type VI  intermediate filament  (IF) protein  that in humans is encoded by the NES  gene. This gene is mapped to 1q23.1. Distribution and expression of nestin in mitotically active cells suggests it plays a role in regulation of the assembly and disassembly of intermediate filaments, which, together with other structural proteins, participate in remodeling of the cell. The role of nestin in dynamic cells, particularly structural organization of the cell, appears strictly regulated by phosphorylation, especially its integration into heterogeneous intermediate filaments together with vimentin or α -internexin. Furthermore, nestin expression has been extensively used as a marker for central nervous system (CNS) progenitor cells in different contexts, based on observations indicating a correlation between nestin expression and this cell type in vivo.

A synthetic peptide corresponding to a sequence at the N-terminus of human Nestin, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

Angiogenesis, Cancer, Cardiovascular, Cell Adhesion Proteins, Cell Type Marker, Developmental Biology, Intermediate Filaments, Intracellular, Invasion/Microenvironment, Lineage Markers, Lineage Specification, Neural Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Stem Cells, Tumor Antigens, Tumor Biomarkers

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity) . .

1. Guérette D, Khan PA, Savard PE, Vincent M (2007) . Molecular evolution of type VI intermediate filament proteins. BMC Evol. Biol. 7: 164. 2. Michalczyk K, Ziman M (April 2005) . Nestin structure and predicted function in cellular cytoskeletal organisation. Histol. Histopathol. 20 (2) : 665–71.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.