Anti-MGA Antibody Picoband® Fluoro647 Conjugated

Mga  is a DNA-binding  protein  that activates the  expression  of several important  virulence  genes  in  Streptococcus pyogenes  (group A  Streptococcus, GAS) in response to changing environmental conditions. It had been found that the mouse transcription factor Max interacted with  Mga.  Coimmunoprecipitation analysis confirmed that Max and  Mga  interacted in transfected HEK293 cells. EMSA revealed that  Mga  required Max for binding to the E-box sequence CACGTG. The isolated T-box of  Mga  bound the brachyury T-box-binding site in the absence of Max.  Mga  repressed transcription of a reporter driven from a T-box-binding site, but coexpression of  Mga  with Max caused transcriptional activation from the T-box-binding site. Expression of  Mga  with Max, but not  Mga  alone, activated transcription from a reporter containing the E-box site.

Functions as a dual-specificity transcription factor, regulating the expression of both MAX-network and T-box family target genes. Functions as a repressor or an activator. Binds to 5'-AATTTCACACCTAGGTGTGAAATT-3' core sequence and seems to regulate MYC-MAX target genes. Suppresses transcriptional activation by MYC and inhibits MYC-dependent cell transformation. Function activated by heterodimerization with MAX. This heterodimerization serves the dual function of both generating an E-box-binding heterodimer and simultaneously blocking interaction of a corepressor (By similarity) . .

1. Hurlin, P. J., Steingrimsson, E., Copeland, N. G., Jenkins, N. A., Eisenman, R. N. Mga, a dual-specificity transcription factor that interacts with Max and contains a T-domain DNA-binding motif. EMBO J. 18: 7019-7028, 1999. Note: Erratum: EMBO J. 19: 3841 only, 2000. 2. McIver KS, Myles RL (March 2002) . Two DNA-binding domains of Mga are required for virulence gene activation in the group A streptococcus. Mol. Microbiol. 43 (6) : 1591–601.