Anti-MDMX/MDM4 Antibody Picoband® Fluoro488 Conjugated

Protein Mdm4?is a?protein?that in humans is encoded by the?MDM4?gene. This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.?

A synthetic peptide corresponding to a sequence at the N-terminus of human MDMX, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Apoptosis, Cancer, Cell Biology, Cell Cycle, Cell Death, Epigenetics and Nuclear Signaling, Metabolism, Metabolism Processes, Nuclear, Nucleus, Pathways and Processes

Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. .

1. Entrez Gene: MDM4 Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse) . 2. Kadakia M, Brown TL, McGorry MM, Berberich SJ (Dec 2002) . MdmX inhibits Smad transactivation. Oncogene21 (57) : 8776–85. 3. Shvarts A, Bazuine M, Dekker P, Ramos YF, Steegenga WT, Merckx G, van Ham RC, van der Houven van Oordt W, van der Eb AJ, Jochemsen AG (Sep 1997) . Isolation and identification of the human homolog of a new p53-binding protein, Mdmx. Genomics 43 (1) : 34–42.