Anti-Filaggrin/FLG Antibody Picoband®

In humans, profillagrin is encoded by the?FLG?gene, which is part of the S100 fused-type protein (SFTP) family within the?epidermal differentiation complex?on?chromosome 1q21. The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.

Expressed in skin, thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland and trachea, but not detected in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal gland (PubMed:19384417) . In the skin, mainly expressed in stratum granulosum of the epidermis (PubMed:1429717) (PubMed:19384417) . .

Cell Type Markers, Class I, Cytoskeleton, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Intermediate Filaments, Signal Transduction, Tags & Cell Markers

Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

1. Kypriotou, Magdalini; Huber, Marcel; Hohl, Daniel (September 2012) .The human epidermal differentiation complex: cornified envelope precursors, S100 proteins and the ‘fused genes’ family. Experimental Dermatology 21 (9) : 643–649. 2. Markova, NG; Marekov, LN; Chipev, CC; Gan, SQ; Idler, WW; Steinert, PM (January 1993) . Profilaggrin is a major epidermal calcium-binding protein.. Molecular and Cellular Biology 13 (1) : 613–25.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Cytoplasmic granule . In the stratum granulosum of the epidermis, localized within keratohyalin granules (PubMed:1429717) . In granular keratinocytes and in lower corneocytes, colocalizes with calpain-1/CAPN1 (PubMed:21531719) . .