Anti-Smac/Diablo Antibody Picoband®

Diablo homolog  (DIABLO), also referred to as  second mitochondria-derived activator of caspases  or  SMAC, is a  mitochondrial  protein  that in humans is encoded by the  DIABLO  (  IAP  binding protein with low pI)   gene  on chromosome 12. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis.   A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.

E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239) . Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac/Diablo.

Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. .

Apoptosis, Cancer, Cell Biology, Cell Death, Invasion/Microenvironment, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Receptors

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP) . Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. .

1. Entrez Gene: DIABLO diablo homolog (Drosophila) . 2. McNeish IA, Bell S, McKay T, Tenev T, Marani M, Lemoine NR (Jun 2003) . Expression of Smac/DIABLO in ovarian carcinoma cells induces apoptosis via a caspase-9-mediated pathway. Experimental Cell Research 286 (2) : 186–98. 3. Yu J, Wang P, Ming L, Wood MA, Zhang L (Jun 2007) . SMAC/Diablo mediates the proapoptotic function of PUMA by regulating PUMA-induced mitochondrial events. Oncogene 26 (29) : 4189–98.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.