Anti-PGP9.5/UCHL1 Antibody Picoband® Fluoro488 Conjugated
Product Specifications
Background
UCH-L1, also known as PGP9.5, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is abundantly present in all neurons (accounts for 1-2% of total brain protein), expressed specifically in neurons and testis/ovary. The catalytic triad of UCH-L1 contains a cysteine at position 90, an aspartate at position 176, and a histidine at position 161 that are responsible for its hydrolase activity.
Synonyms
Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1;3.4.19.12;6.-.-.-; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1
Gene Name
UCHL1
Gene ID
7345
UniProt
P09936
Host
Rabbit
Reactivity
Human, Mouse, Rat
Cross Reactivity
No cross-reactivity with other proteins
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PGP9.5, different from the related mouse and rat sequences by two amino acids.
Clonality
Polyclonal
Tissue Specificity
Found in neuronal cell bodies and processes throughout the neocortex (at protein level) . Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. .
Applications
Flow Cytometry
Field of Research
Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Ubiquitin & Ubiquitin Like Modifiers
Purification
Immunogen affinity purified.
Form
Liquid
Function
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. .
References & Citations
1. Entrez Gene: UCHL1 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) . 2. Das C, Hoang QQ, Kreinbring CA, Luchansky SJ, Meray RK, Ray SS, Lansbury PT, Ringe D, Petsko GA (Mar 2006) . Structural basis for conformational plasticity of the Parkinson's disease-associated ubiquitin hydrolase UCH-L1. Proceedings of the National Academy of Sciences of the United States of America 103 (12) : 4675–80. 3. Doran JF, Jackson P, Kynoch PA, Thompson RJ (Jun 1983) . Isolation of PGP 9.5, a new human neurone-specific protein detected by high-resolution two-dimensional electrophoresis. Journal of Neurochemistry 40 (6) : 1542–7.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
24824 MW
Specificity
No cross reactivity with other proteins.
Applications Notes
6
Gene Name Synonym
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Subcellular Location
Cytoplasm . Endoplasmic reticulum membrane ; Lipid-anchor . About 30% of total UCHL1 is associated with membranes in brain.
Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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