Anti-TRPM8 Antibody Picoband® Fluoro647 Conjugated

Transient receptor potential cation channel subfamily M member 8 (TRPM8), also known as the cold and menthol receptor 1 (CMR1), is a protein that in humans is encoded by the TRPM8 gene. TRPM8 is an ion channel, upon activation it allows the entry of Na+ (sodium) and Ca2+ (calcium) ions to the cell that leads to depolarization and the generation of an action potential. The signal is conducted from primary afferents (type C- and A-delta) eventually leading to the sensation of cold and cold pain. The TRPM8 protein is expressed in sensory neurons, and it is activated by cold temperatures and cooling agents, such as menthol and icilin whereas WS-12 and CPS-369 are the most selective agonist of TRPM8.

Transient receptor potential cation channel subfamily M member 8; Long transient receptor potential channel 6; LTrpC-6; LTrpC6; Transient receptor potential p8; Trp-p8; TRPM8; LTRPC6, TRPP8

A synthetic peptide corresponding to a sequence at the C-terminus of human TRPM8, different from the related mouse sequence by four amino acids, and from the related rat sequence by two amino acids.

Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonist menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. In prostate cancer cells, shows strong inward rectification and high calcium selectivity in contrast to its behavior in normal cells which is characterized by outward rectification and poor cationic selectivity. Plays a role in prostate cancer cell migration (PubMed:25559186) . Isoform 2 and isoform 3 negatively regulate menthol- and cold-induced channel activity by stabilizing the closed state of the channel. .

1. Andersen HH, Møller G, Winter R, Eskelund P, Arendt-Nielsen L (Aug 2013) . A review of topical high-concentration L-menthol as a translational model of cold allodynia and hyperalgesia. European Journal of Pain 18 (3) : 315–325. 2. Sherkheli M.A.; et al. (2007) . Selective TRPM8 agonists: a novel group of neurophathic analgesics. FEBS Journal274 (s1) : 232. 3. Sherkheli M.A., G G, Ak VE, Jf D, H H (October 2008) . Menthol derivative WS-12 selectively activates transient receptor potential melastatin-8 (TRPM8) ion channels. Pak J Pharm Sci 21 (4) : 370–8.

Cell membrane; Multi-pass membrane protein. Membrane raft. Endoplasmic reticulum membrane. Localizes to membrane rafts but is also located in the cell membrane outside of these regions where channel response to cold is enhanced compared to membrane rafts (By similarity) . Located in the endoplasmic reticulum in prostate cancer cells. .