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Anti-DNA polymerase eta/POLH Antibody Picoband® Fluoro647 Conjugated

Product Specifications

Background

DNA polymerase eta (Pol η), is a protein that in humans is encoded by the POLH gene. This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-ed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.

Synonyms

DNA polymerase eta;2.7.7.7; RAD30 homolog A; Xeroderma pigmentosum variant type protein; POLH; RAD30, RAD30A, XPV

Gene Name

POLH

Gene ID

5429

UniProt

Q9Y253

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human POLH recombinant protein (Position: A157-R361) . Human POLH shares 93.6% amino acid (aa) sequence identity with mouse POLH.

Clonality

Polyclonal

Tissue Specificity

Major component of lens fiber gap junctions.

Applications

Flow Cytometry

Field of Research

DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Form

Liquid

Function

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci. .

References & Citations

1. Entrez Gene: POLH polymerase (DNA ed), eta. 2. Johnson RE, Kondratick CM, Prakash S, Prakash L (July 1999) . hRAD30 mutations in the variant form of xeroderma pigmentosum. Science 285 (5425) : 263–5. 3. Masutani C, Kusumoto R, Yamada A, Dohmae N, Yokoi M, Yuasa M, Araki M, Iwai S, Takio K, Hanaoka F (June 1999) . The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase eta. Nature 399 (6737) : 700–4.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

78413 MW

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

DNA polymerase eta

Subcellular Location

Nucleus . Accumulates at replication forks after DNA damage.

Protein Name

DNA polymerase eta

Isotype

Rabbit IgG

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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