Anti-Phospholipase A2/PLA2G4A Antibody Picoband® Fluoro488 Conjugated

PLA2G4A (Phospholipase A2, Group IVA), is an enzyme that in humans is encoded by the PLA2G4A gene. Tay et al. (1995) mapped the PLA2G4A gene to rat chromosome 13 by PCR-based intercross genotyping and to human 1q25 by fluorescence in situ hybridization. By site-ed mutagenesis and biochemical analysis of the recombinant protein, Sharp et al. (1994) determined that ser228 participates in the catalytic mechanism of cPLA2 and that both the phospholipase A2 and the lysophospholipase activities are catalyzed by the same active site residue (s) . PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands (Skorecki, 1995) .

Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA; Phospholipase A2;3.1.1.4; Phosphatidylcholine 2-acylhydrolase; Lysophospholipase;3.1.1.5; PLA2G4A; CPLA2, PLA2G4

A synthetic peptide corresponding to a sequence at the C-terminus of human PLA2G4A, different from the related mouse and rat sequences by three amino acids.

Atherosclerosis, Cancer, Cardiovascular, Heart Disease, Immunology, Innate Immunity, Lipid and Lipoprotein Metabolism, Lipid Signaling, Macrophage/Inflammation, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Signaling Pathway, Vascular Inflammation

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

1. Sharp, J. D., Pickard, R. T., Chiou, X. G., Manetta, J. V., Kovacevic, S., Miller, J. R., Varshavsky, A. D., Roberts, E. F., Strifler, B. A., Brems, D. N., Kramer, R. M. Serine 228 is essential for catalytic activities of 85-kDa cytosolic phospholipase A2. J. Biol. Chem. 269: 23250-23254, 1994. 2. Skorecki, K. L. Personal Communication. Toronto, Canada 4/19/1995. 3. Tay, A., Simon, J. S., Squire, J., Hamel, K., Jacob, H. J., Skorecki, K. Cytosolic phospholipase A2 gene in human and rat: chromosomal localization and polymorphic markers. Genomics 26: 138-141, 1995.