Anti-PU.1/Spi1 Antibody

Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene. This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

E.coli-derived human PU.1/Spi1 recombinant protein (Position: E18-K196) . Human PU.1/Spi1 shares 84% and 84.4% amino acid (aa) sequence identity with mouse and rat PU.1/Spi1, respectively.

Adaptive Immunity, B Cells, DNA/RNA, Domain Families, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Macrophage/Inflammation, Non-CD, RNA Processing, Splicing, Transcription, Transcription Factors

Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity) . .

1. Entrez Gene: SPI1 spleen focus forming virus (SFFV) proviral integration oncogene spi1. 2. Hallier M, Lerga A, Barnache S, Tavitian A, Moreau-Gachelin F (February 1998) . The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS. J. Biol. Chem. 273 (9) : 4838–42. 3. Ray D, Culine S, Tavitain A, Moreau-Gachelin F (July 1990) . The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors. Oncogene 5 (5) : 663–8.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.