Anti-Tissue Factor/F3 Antibody Picoband®

Tissue factor also called platelet tissue factor, ?factor III, or?CD142. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.

E.coli-derived mouse Tissue Factor recombinant protein (Position: A29-A294) . Mouse Tissue Factor shares 62.2% and 82.1% amino acid (aa) sequence identity with human and rat Tissue Factor, respectively.

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

1. Entrez Gene: F3 coagulation factor III (thromboplastin, tissue factor) . 2. Bogdanov VY, Balasubramanian V, Hathcock J, Vele O, Lieb M, Nemerson Y (April 2003) . Alternatively spliced human tissue factor: a circulating, soluble, thrombogenic protein. Nat. Med. 9 (4) : 458–62. 3. Mackman N (1995) . Regulation of the tissue factor gene. FASEB J. 9 (10) : 883–9.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.