Anti-KIF3A Antibody Picoband®

Kinesin-like protein KIF3A is a protein that in humans is encoded by the KIF3A gene. KIF3A is one subunit of the heterotrimeric motor protein, kinesin-2, that was initially isolated from sea urchin egg/embryo cytosol using microtubule affinity purification. This motor consists of two kinesin-related subunits (called KIF3A and KIF3B or 3C in vertebrates) and an associated protein (KAP3), and it transports protein complexes, nucleic acids and organelles towards the plus ends of microtubule tracks within cells. Work done in a broad range of eukaryotic cells has revealed that heterotrimeric kinesin-2 is the primary motor protein driving the intra-flagellar transport of tubulins and other axonemal building blocks from the base of the ciliary/flagellar axoneme to their site of assembly at the distal tips. This process is required for cilium assembly/maintenance and cilium-based signalling which play key roles in various cell and developmental processes. For example, in vertebrate embryos, kinesin-2 function is required for cilia-dependent nodal flow and the development of left-right asymmetry.

Cornea. Increased expression in the stroma of keratoconus corneas. Also detected in trachea, and in low levels, in intestine, skeletal muscle, ovary, lung and putamen. .

Adapters, Cytoskeleton, Cytoskeleton/ECM, Motor Proteins, Neuroscience, Neurotransmitter, Protein Trafficking, Signal Transduction, Signaling Pathways, Stem Cells, Surface Molecules, Transporters, Vesicle Transport

Microtubule-based anterograde translocator for membranous organelles. Plus end-ed microtubule sliding activity in vitro. Plays a role in primary cilia formation (By similarity) . .

1. Cole DG, Chinn SW, Wedaman KP, Hall K, Vuong T, Scholey JM (November 1993) . Novel heterotrimeric kinesin-related protein purified from sea urchin eggs. Nature 366 (6452) : 268–70. 2. Nonaka S, Tanaka Y, Okada Y, Takeda S, Harada A, Kanai Y, Kido M, Hirokawa N (December 1998) . Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein. Cell 95 (6) : 829–37. 3. Rosenbaum JL, Witman GB (November 2002) . Intraflagellar transport. Nat. Rev. Mol. Cell Biol. 3 (11) : 813–25.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.