Anti-GRP94/HSP90B1 Antibody Picoband® Fluoro647 Conjugated
Product Specifications
Background
Heat shock protein 90kDa beta member 1 (HSP90B1), known as endoplasmin, or GRP94, is a chaperone protein that in humans is encoded by the HSP90B1 gene. It is mapped to chromosome 12q23.3. This gene encodes a member of a family of adenosine triphosphate (ATP) -metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation.
Synonyms
Endoplasmin;94 kDa glucose-regulated protein; GRP-94; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; gp96 homolog; HSP90B1; GRP94, TRA1
Gene Name
HSP90B1
Gene ID
7184
UniProt
P14625
Host
Rabbit
Reactivity
Human, Mouse, Rat
Cross Reactivity
No cross-reactivity with other proteins
Immunogen
E.coli-derived human GRP94 recombinant protein (Position: R43-H221) . Human GRP94 shares 99.4% and 98.9% amino acid (aa) sequence identity with mouse and rat GRP94, respectively.
Clonality
Polyclonal
Tissue Specificity
Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues. .
Applications
Flow Cytometry
Field of Research
Cancer, Cancer Metabolism, ER Proteins, Metabolism, Metabolism Processes, Organelles, Pathways and Processes, Protein Trafficking, Response To Hypoxia, Signal Transduction, Subcellular Markers, Tags & Cell Markers
Purification
Immunogen affinity purified.
Form
Liquid
Function
Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity) . Functions in endoplasmic reticulum associated degradation (ERAD) . Has ATPase activity. .
References & Citations
1. Chen B, Piel WH, Gui L, Bruford E, Monteiro A (December 2005) . The HSP90 family of genes in the human genome: insights into their divergence and evolution. Genomics 86 (6) : 627–37. 2. Maki RG, Old LJ, Srivastava PK (August 1990) . Human homologue of murine tumor rejection antigen gp96: 5'-regulatory and coding regions and relationship to stress-induced proteins. Proc. Natl. Acad. Sci. U.S.A.87 (15) : 5658–62. 3. Randow F, Seed B (2001) . Endoplasmic reticulum chaperone gp96 is required for innate immunity but not cell viability.. Nat. Cell Biol. 3 (10) : 891–6.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
92469 MW
Specificity
No cross reactivity with other proteins.
Applications Notes
6
Gene Name Synonym
Endoplasmin
Subcellular Location
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Sequence Similarities
Contains 1 HMG box DNA-binding domain.
Protein Name
Endoplasmin
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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