What sizes are available for Anti-Cofilin/CFL1 Antibody Picoband® Fluoro594 Conjugated?

Anti-Cofilin/CFL1 Antibody Picoband® Fluoro594 Conjugated is available in the following size(s): 100 µg/Vial.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

Anti-Cofilin/CFL1 Antibody Picoband® Fluoro594 Conjugated

Q: What is the catalog number for Anti-Cofilin/CFL1 Antibody Picoband® Fluoro594 Conjugated?

The catalog number for Anti-Cofilin/CFL1 Antibody Picoband® Fluoro594 Conjugated is PB9620-Fluoro594.

Product Specifications

Background

Cofilin, a member of the ADF/cofilin family, is actually a protein with 70% sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments. The protein is known to sever actin filaments by creating more positive ends on filament fragments, it can cause depolymerization at the minus end of filaments, thereby preventing their reassembly. As a long-lasting in vivo effect, cofilin recycles older ADP-F-actin, helping cell to maintain ATP-G-actin pool for sustained motility. pH, phosphorylation and phosphoinositides regulate cofilin’s binding and associating activity with actin.

Synonyms

Cofilin-1;18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform; CFL1; CFL

Gene Name

CFL1

Gene ID

1072

UniProt

P23528

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Widely distributed in various tissues.

Applications

Flow Cytometry

Field of Research

Invasion/Microenvironment, Microfilaments, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction, Vasculature

Purification

Immunogen affinity purified.

Form

Liquid

Function

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. .

References & Citations

1. Cooper, G. M. and R. E. Hausman. The Cell: A Molecular Approach, 3rd ed. Washington DC: ASM Press 2004 pp.436-440. 2. Ichetovkin I, Han J, Pang KM, Knecht DA, Condeelis JS (April 2000) . Actin filaments are severed by both native and recombinant dictyostelium cofilin but to different extents. Cell Motil. Cytoskeleton 45 (4) : 293–306. 3. Lappalainen P, Drubin DG (July 1997) . Cofilin promotes rapid actin filament turnover in vivo.Nature 388 (6637) : 78–82.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

18502 MW

Specificity

No cross reactivity with other proteins.

Applications Notes

Gene Name Synonym

Cofilin-1

Subcellular Location

Nucleus matrix . Cytoplasm, cytoskeleton . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Peripheral membrane protein ; Cytoplasmic side . Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.

Sequence Similarities

Contains 1 HMG box DNA-binding domain.

Protein Name

Cofilin-1

Isotype

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

Histone H1 (AE-4), CF640R conjugate, 0.1mg/mL

BNC400096-100 1x 100 µL