Anti-Livin/BIRC7 Antibody Picoband® Fluoro647 Conjugated

Baculoviral IAP repeat-containing protein 7 is a protein that in humans is encoded by the BIRC7 gene. This protein is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) and a RING finger motif. Its gene is mapped to 20q13.3. It has got 280- and 298- amino acids. The protein is highly expressed in melanomas while lowly expressed in some lymphomas, fetal kidney, fetal liver, testis and thymus. BIRC7 plays a vital role in blocking apoptosis induced.

Baculoviral IAP repeat-containing protein 7;6.3.2.-; Kidney inhibitor of apoptosis protein; KIAP; Livin; Melanoma inhibitor of apoptosis protein; ML-IAP; RING finger protein 50; Baculoviral IAP repeat-containing protein 7 30kDa subunit; Truncated livin; p30-Livin; tLivin; BIRC7; KIAP, LIVIN, MLIAP, RNF50; UNQ5800/PRO19607/PRO21344

Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes.

Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. Isoform 2 protects against natural killer (NK) cell killing whereas isoform 1 augments killing. .

1. Entrez Gene: BIRC7 baculoviral IAP repeat-containing 7 (livin) . 2. Kasof GM, Gomes BC (May 2001) . Livin, a novel inhibitor of apoptosis protein family member. J Biol Chem 276 (5) : 3238–46. 3. Lin JH, Deng G, Huang Q, Morser J (Feb 2001) . KIAP, a novel member of the inhibitor of apoptosis protein family. Biochem Biophys Res Commun 279 (3) : 820–31.

Nucleus . Cytoplasm . Golgi apparatus . Nuclear, and in a filamentous pattern throughout the cytoplasm. Full-length livin is detected exclusively in the cytoplasm, whereas the truncated form (tLivin) is found in the peri-nuclear region with marked localization to the Golgi apparatus; the accumulation of tLivin in the nucleus shows positive correlation with the increase in apoptosis.