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Anti-Osteopontin/SPP1 Antibody Picoband®

Boster Bio Anti-Osteopontin/SPP1 Antibody catalog # RP1080. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Osteopontin (OPN), also known as secreted phosphoprotein 1 (SPP1), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1) . The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. And the encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. Also, this protein is a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.

Synonyms

Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin; SPP1; BNSP, OPN; PSEC0156

Gene Name

SPP1

Gene ID

6696

UniProt

P10451

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Osteopontin, different from the related mouse sequence by eight amino acids, and from the related rat sequence by seven amino acids.

Clonality

Polyclonal

Tissue Specificity

Bone. Found in plasma.

Applications

ELISA, WB

Field of Research

Angiogenesis, Cancer, Cardiovascular, Cytoskeleton/ECM, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells, Structures, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

References & Citations

1. Entrez Gene: SPP1 secreted phosphoprotein 1. 2. Reinholt FP, Hultenby K, Oldberg A, Heinegård D (June 1990) . Osteopontin--a possible anchor of osteoclasts to bone. Proc. Natl. Acad. Sci. U.S.A. 87 (12) : 4473–5. 3. Wang KX, Denhardt DT (2008) . Osteopontin: role in immune regulation and stress responses. Cytokine Growth Factor Rev. 19 (5-6) : 333–45.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

35423 MW

Observed Molecular Weight

66 kDa, 25-55 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Osteopontin

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the osteopontin family.

Protein Name

Osteopontin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

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