Anti-EBAG9 Antibody Picoband®

Receptor-binding cancer antigen expressed on SiSo cells is a protein that in humans is encoded by the EBAG9 gene. This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. And the encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10.

Receptor-binding cancer antigen expressed on SiSo cells; Cancer-associated surface antigen RCAS1; Estrogen receptor-binding fragment-associated gene 9 protein; EBAG9; RCAS1

E.coli-derived human EBAG9 recombinant protein (Position: R31-S213) . Human EBAG9 shares 97.8% and 94.5% amino acid (aa) sequence identity with mouse and rat EBAG9, respectively.

Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.

May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1- beta converting enzyme (ICE) -like proteases. .

1. Entrez Gene: EBAG9 estrogen receptor binding site associated, antigen, 9. 2. Ikeda K; Sato M; Tsutsumi O et al. (2000) . Promoter analysis and chromosomal mapping of human EBAG9 gene. Biochem. Biophys. Res. Commun. 273 (2) : 654–60. 3. Tsuneizumi M; Emi M; Nagai H et al. (2002) . Overrepresentation of the EBAG9 gene at 8q23 associated with early-stage breast cancers. Clin. Cancer Res. 7 (11) : 3526–32.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Golgi apparatus membrane ; Single-pass type III membrane protein . According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.