Anti-SAP97/DLG1 Antibody Picoband®

Disks large homolog 1 (DLG1), also known as synapse-associated protein 97 or SAP97, is a protein that in humans is encoded by the SAP97 gene. SAP97 is expressed throughout the body in epithelial cells, including the kidney and brain. There is some evidence that SAP97 regulates cell-to-cell adhesion during cell death, and may interact with HPV. In the brain, SAP97's function is involved in the trafficking of transmembrane receptors from the ER to the plasma membrane. SAP97's function has been investigated by reducing its expression by knockout or increasing its expression heterologously. Mice in which the SAP97 gene has been knocked out die perinatally, have a cleft palate, and deficiencies in renal function. Overexpression of SAP97 in mammalian neurons leads to increased synaptic strength.

E.coli-derived human SAP97 recombinant protein (Position: M1-A165) . Human SAP97 shares 89.7% and 84.2% amino acid (aa) sequence identity with mouse and rat SAP97, respectively.

Abundantly expressed in atrial myocardium (at protein level) . Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level) . Widely expressed, with isoforms displaying different expression profiles. .

Adapters, Cell Type Marker, Host-Virus Interaction, Interspecies Interaction, Intracellular Signaling, Microbiology, Neuron Marker, Neuroscience, Neurotransmission, Synapse Marker

Essential multidomain scaffolding protein required for normal development (By similarity) . Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. .

1. Müller BM; Kistner U; Veh RW et al. (March 1995) . Molecular characterization and spatial distribution of SAP97, a novel presynaptic protein homologous to SAP90 and the Drosophila discs-large tumor suppressor protein. J. Neurosci. 15 (3 Pt 2) : 2354–66. 2. umbaugh G, Sia GM, Garner CC, Huganir RL (June 2003) . Synapse-associated protein-97 isoform-specific regulation of surface AMPA receptors and synaptic function in cultured neurons. J. Neurosci. 23 (11) : 4567–76. 3. Sans N, Racca C, Petralia RS, Wang YX, McCallum J, Wenthold RJ (October 2001) . Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway. J. Neurosci. 21 (19) : 7506–16.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Membrane ; Peripheral membrane protein . Basolateral cell membrane . Endoplasmic reticulum membrane . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell junction, synapse. Cell membrane, sarcolemma. Colocalizes with EPB41 at regions of intercellular contacts. Basolateral in epithelial cells. May also associate with endoplasmic reticulum membranes. Mainly found in neurons soma, moderately found at postsynaptic densities (By similarity) . .