Anti-PKLR Antibody Picoband® Fluoro647 Conjugated
Product Specifications
Background
Pyruvate kinase isozymes R/L is an enzyme that in humans is encoded by the PKLR gene. It is mapped to 1q21. The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA) . Multiple transcript variants encoding different isoforms have been found for this gene.
Synonyms
Pyruvate kinase PKLR;2.7.1.40; Pyruvate kinase 1; Pyruvate kinase isozymes L/R; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase; PKLR; PK1, PKL
Gene Name
PKLR
Gene ID
5313
UniProt
P30613
Host
Rabbit
Reactivity
Human, Mouse, Rat
Cross Reactivity
No cross-reactivity with other proteins
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PKLR, different from the related mouse sequence by one amino acid, and identical to the rat sequence.
Clonality
Polyclonal
Tissue Specificity
Ubiquitous; expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.
Applications
Flow Cytometry
Field of Research
Cancer, Cancer Metabolism, Carbohydrate Metabolism, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Pathways and Processes, Signal Transduction
Purification
Immunogen affinity purified.
Form
Liquid
Function
Plays a key role in glycolysis. .
References & Citations
1. Entrez Gene: PKLR pyruvate kinase, liver and RBC. 2. Tani K, Fujii H, Tsutsumi H, Sukegawa J, Toyoshima K, Yoshida MC, Noguchi T, Tanaka T, Miwa S (Apr 1987) . Human liver type pyruvate kinase: cDNA cloning and chromosomal assignment. Biochem Biophys Res Commun 143 (2) : 431–8.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
61830 MW
Specificity
No cross reactivity with other proteins.
Applications Notes
6
Gene Name Synonym
Pyruvate kinase PKLR
Subcellular Location
Nucleus. Nucleus, nucleolus. Chromosome, telomere. Chromosome, centromere, kinetochore. Localizes in nucleoli, at telomere speckles and to the outer plate of kinetochores. Localization to the kinetochore is mediated by its central region and depends on NDC80 and CENPE.
Sequence Similarities
Belongs to the pyruvate kinase family.
Protein Name
Pyruvate kinase PKLR
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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