Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Anti-BAK/BAK1 Antibody Picoband®

Boster Bio Anti-BAK/BAK1 Antibody Picoband® catalog # PB9484. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene. The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons. By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Synonyms

Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7; BAK1; BAK, BCL2L7, CDN1

Gene Name

BAK1

Gene ID

578

UniProt

Q16611

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human BAK recombinant protein (Position: A22-S211) . Human BAK shares 78.3 % amino acid (aa) sequence identity with mouse BAK.

Clonality

Polyclonal

Tissue Specificity

Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Apoptosis, Cell Biology, Intracellular

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. .

References & Citations

1. Chittenden, T., Harrington, E. A., O'Connor, R., Flemington, C., Lutz, R. J., Evan, G. I., Guild, B. C.Induction of apoptosis by the Bcl-2 homologue Bak.Nature 374: 733-736, 1995. 2. Herberg, J. A., Phillips, S., Beck, S., Jones, T., Sheer, D., Wu, J. J., Prochazka, V., Barr, P. J., Kiefer, M. C., Trowsdale, J.Genomic structure and domain organisation of the human Bak gene.Gene 211: 87-94, 1998. 3. Kiefer, M. C., Brauer, M. J., Powers, V. C., Wu, J. J., Umansky, S. R., Tomei, L. D., Barr, P. J.Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak.Nature 374: 736-739, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

23 kDa

Observed Molecular Weight

23 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Bcl-2 homologous antagonist/killer

Subcellular Location

Mitochondrion membrane ; Single-pass membrane protein .

Sequence Similarities

Belongs to the Bcl-2 family.

Protein Name

Bcl-2 homologous antagonist/killer

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Rat Thymocyte antibody (FITC)
MBS534745-01 2 mg

Rat Thymocyte antibody (FITC)

Ask
View Details
Rat Thymocyte antibody (FITC)
MBS534745-02 5x 2 mg

Rat Thymocyte antibody (FITC)

Ask
View Details
SAR-020106
GW6888-10mg 10 mg

SAR-020106

Ask
View Details
Isocarboxazid 50mg
A17887-50 50 mg

Isocarboxazid 50mg

Ask
View Details
ALG8, NT (ALG8, Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase, Asparagine-linked glycosylation protein 8 homolog, Dol-P-Glc:Glc (1) Man (9) GlcNAc (2) -PP-dolichyl alpha-1,3-glucosyltransferase, Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase)
031793 200 µL

ALG8, NT (ALG8, Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase, Asparagine-linked glycosylation protein 8 homolog, Dol-P-Glc:Glc (1) Man (9) GlcNAc (2) -PP-dolichyl alpha-1,3-glucosyltransferase, Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase)

Ask
View Details
RBP4 Monoclonal Antibody
A10270-100UG 100 µg

RBP4 Monoclonal Antibody

Ask
View Details