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Anti-AGFG1 Antibody Picoband®

Boster Bio Anti-AGFG1 Antibody catalog # RP1071. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Arf-GAP domain and FG repeats-containing protein 1 is aprotein that in humans is encoded by the AGFG1 gene. AGFG1 is located to 2q36.3. The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-ed RNAs. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Synonyms

Arf-GAP domain and FG repeat-containing protein 1; HIV-1 Rev-binding protein; Nucleoporin-like protein RIP; Rev-interacting protein; Rev/Rex activation domain-binding protein; AGFG1; HRB, RAB, RIP

Gene Name

AGFG1

Gene ID

3267

UniProt

P52594

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human AGFG1, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed. .

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Antiviral Signaling, Immune System Diseases, Immunology, Nuclear Import/Export, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Required for vesicle docking or fusion during acrosome biogenesis (By similarity) . May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element- containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication. .

References & Citations

1. Entrez Gene: HRB HIV-1 Rev binding protein. 2. Fritz CC, Zapp ML, Green MR (Sep 1995) . A human nucleoporin-like protein that specifically interacts with HIV Rev.Nature 376 (6540) : 530–3.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

58260 MW

Observed Molecular Weight

58 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Arf-GAP domain and FG repeat-containing protein 1

Subcellular Location

Nucleus. Cytoplasmic vesicle.

Sequence Similarities

Contains 1 Arf-GAP domain.

Protein Name

Arf-GAP domain and FG repeat-containing protein 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

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