Anti-SLUG/SNAI2 Antibody Picoband®

SLUG is also known as SNAI2. This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.

Expressed in most adult human tissues, including spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Not detected in peripheral blood leukocyte. Expressed in the dermis and in all layers of the epidermis, with high levels of expression in the basal layers (at protein level) . Expressed in osteoblasts (at protein level) . Expressed in mesenchymal stem cells (at protein level) . Expressed in breast tumor cells (at protein level) . .

Cancer, Cell Type Marker, Developmental Biology, Domain Families, Epigenetics and Nuclear Signaling, Lineage Markers, Lineage Specification, Nervous System Development, Neural Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Oncoproteins, Oncoproteins/Suppressors, Organogenesis, Stem Cells, Transcription, Transcription Factors, Zinc Finger

Transcriptional repressor that modulates both activator- dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1- induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (By similarity) . Represses BRCA2 expression by binding to its E2- box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis. .

1. Entrez Gene: SNAI2 snail homolog 2 (Drosophila) 2. Cohen ME, Yin M, Paznekas WA, Schertzer M, Wood S, Jabs EW (Oct 1998) . Human SLUG gene organization, expression, and chromosome map location on 8q. Genomics 51 (3) : 468–71. 3. Rhim H, Savagner P, Thibaudeau G, Thiery JP, Pavan WJ (Jan 1998) . Localization of a neural crest transcription factor, Slug, to mouse chromosome 16 and human chromosome 8. Mamm Genome 8 (11) : 872–3.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Nucleus. Cytoplasm. Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions.