What sizes are available for Anti-Smad3 Antibody Picoband® Fluoro647 Conjugated?

Anti-Smad3 Antibody Picoband® Fluoro647 Conjugated is available in the following size(s): 100 µg/Vial.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

Anti-Smad3 Antibody Picoband® Fluoro647 Conjugated

Q: What is the catalog number for Anti-Smad3 Antibody Picoband® Fluoro647 Conjugated?

The catalog number for Anti-Smad3 Antibody Picoband® Fluoro647 Conjugated is PB9396-Fluoro647.

Q: Is Anti-Smad3 Antibody Picoband® Fluoro647 Conjugated in stock?

Anti-Smad3 Antibody Picoband® Fluoro647 Conjugated is currently made to order / not held in immediate stock. Contact us for lead time.

Product Specifications

Background

Mothers against decapentaplegic homolog 3 also known as SMAD family member 3 or SMAD3 is a protein that in humans is encoded by the SMAD3 gene. SMAD3 is a member of the SMAD family of proteins. It is located in 15q22.33. And it mediates the signals from the transforming growth factor beta (TGF-β) super family ligands that regulate cell proliferation, differentiation and death. Based on its essential role in TGF beta signaling pathway, SMAD3 has been related with tumor growth in cancer development.

Synonyms

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

Gene Name

SMAD3

Gene ID

4088

UniProt

P84022

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Smad3, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Applications

Flow Cytometry

Field of Research

Cancer, Cancer Metabolism, Cytoplasmic, Epigenetics and Nuclear Signaling, Metabolism, Metabolism Processes, Nuclear Signaling, Nuclear Signaling Pathways, Pathways and Processes, Response To Hypoxia, Signal Transduction, Signaling Pathway, Stem Cells, TGF Beta

Purification

Immunogen affinity purified.

Form

Liquid

Function

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. .

References & Citations

1. Entrez Gene: SMAD3 SMAD family member 3. 2. Moustakas A, Souchelnytskyi S, Heldin CH (2001) . Smad regulation in TGF-beta signal transduction. J. Cell. Sci. 114 (Pt 24) : 4359–69. 3. Zhang Y, Feng X, We R, Derynck R (September 1996) . Receptor-associated Mad homologues synergize as effectors of the TGF-beta response. Nature 383 (6596) : 168–72.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

48 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

Gene Name Synonym

Mothers against decapentaplegic homolog 3

Subcellular Location

Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane. MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta.