Anti-Glucocorticoid Receptor/NR3C1 Antibody Picoband® Fluoro647 Conjugated

The glucocorticoid receptor (GR, or GCR), also known as NR3C1, is the receptor to which cortisol and other glucocorticoids bind. In humans, the GR protein is encoded by NR3C1 gene which is located on chromosome 5 (5q31) . GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response. Because the receptor gene is expressed in several forms, it has many different (pleiotropic) effects in different parts of the body. The activated GR complex up-regulates the expression of anti-inflammatory proteins in the nucleus or represses the expression of pro-inflammatory proteins in the cytosol (by preventing the translocation of other transcription factors from the cytosol into the nucleus) .

E.coli-derived human NR3C1 recombinant protein (Position: A20-F199) . Human NR3C1 shares 80% and 74% amino acid (aa) sequence identity with mouse and rat NR3C1, respectively.

Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. .

2339, Domain Families, Epigenetics and Nuclear Signaling, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Transcription, Zinc Finger

Receptor for glucocorticoids (GC) . Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression. .

1. Lu NZ, Wardell SE, Burnstein KL, Defranco D, Fuller PJ, Giguere V, Hochberg RB, McKay L, Renoir JM, Weigel NL, Wilson EM, McDonnell DP, Cidlowski JA (2006) . International Union of Pharmacology. LXV. The pharmacology and classification of the nuclear receptor superfamily: glucocorticoid, mineralocorticoid, progesterone, and androgen receptors. Pharmacol Revl 58 (4) : 782–97. 2. Rhen T, Cidlowski JA (October 2005) . Antiinflammatory action of glucocorticoids--new mechanisms for old drugs. N. Engl. J. Med. 353 (16) : 1711–23. 3. Hollenberg SM, Weinberger C, Ong ES, Cerelli G, Oro A, Lebo R, Thompson EB, Rosenfeld MG, Evans RM (1985) . Primary structure and expression of a functional human glucocorticoid receptor cDNA. Nature 318 (6047) : 635–41.