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Anti-APPBP1/NAE1 Antibody Picoband®

Boster Bio Anti-APPBP1/NAE1 Antibody Picoband® catalog # PB9339. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

NAE1 is also known as APPBP1. The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.

Synonyms

NEDD8-activating enzyme E1 regulatory subunit; Amyloid beta precursor protein-binding protein 1, 59 kDa; APP-BP1; Amyloid protein-binding protein 1; Proto-oncogene protein 1; NAE1; APPBP1; HPP1

Gene Name

NAE1

Gene ID

8883

UniProt

Q13564

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human APPBP1 recombinant protein (Position: E181-L534) . Human APPBP1 shares 98% amino acid (aa) sequence identity with mouse APPBP1.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous in fetal tissues. Expressed throughout the adult brain. .

Applications

WB

Field of Research

Cell Biology, Epigenetics and Nuclear Signaling, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ub-Like Proteins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. .

References & Citations

1. Hong BH, et al. Amyloid precursor protein binding protein-1 knockdown reduces neuronal differentiation in fetal neural stem cells. Neuroreport, 2012 Jan 25. 2. Joo Y, et al. Amyloid precursor protein binding protein-1 modulates cell cycle progression in fetal neural stem cells. PLoS One, 2010 Dec 2. 3. Zhong HJ, et al. A metal-based inhibitor of NEDD8-activating enzyme. PLoS One, 2012.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

60246 MW

Observed Molecular Weight

42 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

NEDD8-activating enzyme E1 regulatory subunit

Subcellular Location

Cell membrane . Colocalizes with APP in lipid rafts.

Protein Name

NEDD8-activating enzyme E1 regulatory subunit

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

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