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Anti-Mad2L1 Antibody Picoband® Fluoro594 Conjugated

Product Specifications

Background

Mitotic spindle assembly checkpoint protein MAD2A is a protein that in humans is encoded by the MAD2L1 gene. This gene belongs to the MAD2 family. It is mapped to 4q27. MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. It is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment.

Synonyms

Mitotic spindle assembly checkpoint protein MAD2A; HsMAD2; Mitotic arrest deficient 2-like protein 1; MAD2-like protein 1; MAD2L1; MAD2

Gene Name

MAD2L1

Gene ID

4085

UniProt

Q13257

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Mad2L1 recombinant protein (Position: A2-D205) . Human Mad2L1 shares 94% amino acid (aa) sequence identity with mouse Mad2L1.

Clonality

Polyclonal

Tissue Specificity

Expressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level) . Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. .

Applications

Flow Cytometry

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Division, Nucleus, Spindle, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Form

Liquid

Function

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore- spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. .

References & Citations

1. Cahill, D. P., da Costa, L. T., Carson-Walter, E. B., Kinzler, K. W., Vogelstein, B., Lengauer, C.Characterization of MAD2B and other mitotic spindle checkpoint genes. Genomics 58: 181-187, 1999. 2. Entrez Gene: MAD2L1 MAD2 mitotic arrest deficient-like 1 (yeast)

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

24 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Mitotic spindle assembly checkpoint protein MAD2A

Subcellular Location

Nucleus. Chromosome, centromere, kinetochore. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Recruited by MAD1L1 to unattached kinetochores (Probable) . Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD. .

Sequence Similarities

Belongs to the MAD2 family.

Protein Name

Mitotic spindle assembly checkpoint protein MAD2A

Isotype

Rabbit IgG

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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