Anti-IKB alpha/NFKBIA Antibody Picoband®

NFKBIA, also called IKBA or MAD-3, is one member of a family of cellular proteins that function to inhibit the NF-κ B transcription factor. It is mapped to 14q13.2. NFKBIA inhibits NF-κ B by masking the nuclear localization signals (NLS) of NF-κ B proteins and keeping them sequestered in an inactive state in the cytoplasm. It moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. The effect of the nonpathogenic bacteria is specific to the SCF complex substrates CTNNB1 and NFKBIA. This may help to explain the beneficial effects of treatment of inflammatory bowel disease with nonpathogenic probiotic enteric organisms. In addition, NFKBIA blocks the ability of NF-κ B transcription factors to bind to DNA, which is required for NF-κ B's proper functioning.

NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA, MAD3, NFKBI

E.coli-derived human IKB alpha recombinant protein (Position: Q3-Q112) . Human IKB alpha shares 87% and 86% amino acid (aa) sequence identity with mouse and rat IKB alpha, respectively.

Cancer, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, NFKB Pathway, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, TLR Signaling

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. .

1. Jacobs MD, Harrison SC (1998) . Structure of an IkappaBalpha/NF-kappaB complex. Cell 95 (6) : 749–58. 2. Neish, A. S.., Gewirtz, A. T., Zeng, H., Young, A. N., Hobert, M. E., Karmali, V., Rao, A. S., Madara, J. L. Prokaryotic regulation of epithelial responses by inhibition of I-kappa-B-alpha ubiquitination. Science 289: 1560-1563, 2000. 3. Verma IM, Stevenson JK, Schwarz EM, Van Antwerp D, Miyamoto S (1995) . Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation. Genes Dev.9 (22) : 2723–35.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.