Anti-Nkx2.5/NKX2-5 Antibody Picoband® Fluoro488 Conjugated

Homeobox protein Nkx-2.5, also known as NKX2E or CSX is a protein that in humans is encoded by the NKX2-5 gene. It is mapped to 5q35.1. Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development. Nkx2.5 and Tbx5 ly bound to the promoter of the gene encoding cardiac-specific natriuretic peptide precursor type A (NPPA) in tandem, and both transcription factors showed synergistic activation. The cardiac homeobox protein Nkx2.5 is essential in cardiac development, and mutations in CSX (which encodes Nkx2.5) cause various congenital heart malformations.

A synthetic peptide corresponding to a sequence in the middle region of human Nkx2.5, different from the related mouse and rat sequences by five amino acids.

Cardiogenesis, Cardiovascular, Developmental Biology, Lineage Markers, Lineage Specification, Mesenchymal Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Stem Cells, Transcription Factors/Regulators

Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity) . It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity) . It is required for spleen development. .

1. Hiroi, Y., Kudoh, S., Monzen, K., Ikeda, Y., Yazaki, Y., Nagai, R., Komuro, I. Tbx5 associates withNkx2-5 and synergistically promotes cardiomyocyte differentiation. Nature Genet. 28: 276-280, 2001. 2. Shiojima, I., Komuro, I., Inazawa, J., Nakahori, Y., Matsushita, I., Abe, T., Nagai, R., Yazaki, Y.Assignment of cardiac homeobox gene CSX to human chromosome 5q34. Genomics 27: 204-206, 1995. 3. Turbay D, Wechsler SB, Blanchard KM, Izumo S (January 1997) . Molecular cloning, chromosomal mapping, and characterization of the human cardiac-specific homeobox gene hCsx. Mol Med 2 (1) : 86–96.