Anti-CARD4/NOD1 Antibody Picoband® Fluoro647 Conjugated
Product Specifications
Background
Nucleotide-binding oligomerization domain-containing protein 1, also known as CARD4, is a protein receptor that in humans is encoded by the NOD1 gene. NOD1 is a member of NOD-like receptor protein family and is a close relative of NOD2. NOD1 is mapped to 7p14.3. It recognizes bacterial molecules and stimulates an immune reaction. NOD1 protein contains a caspase recruitment domain (CARD) . This gene is an intracellular pattern recognition receptor, which is similar in structure to resistant proteins of plants, and mediates innate and acquired immunity by recognizing bacterial molecules containing D-glutamyl-meso-diaminopimelic acid (iE-DAP) moiety. What wore, it has been shown that NOD1 can sense cytosolic microbial products by monitoring the activation state of small Rho GTPases.
Synonyms
Nucleotide-binding oligomerization domain-containing protein 1; Caspase recruitment domain-containing protein 4; NOD1; CARD4
Gene Name
NOD1
Gene ID
10392
UniProt
Q9Y239
Host
Rabbit
Reactivity
Human, Rat
Cross Reactivity
No cross-reactivity with other proteins
Immunogen
E.coli-derived human CARD4 recombinant protein (Position: M1-M160) . Human CARD4 shares 82% amino acid (aa) sequence identity with mouse CARD4.
Clonality
Polyclonal
Tissue Specificity
Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.
Applications
Flow Cytometry
Field of Research
Apoptosis, Caspases, Cell Biology, Intracellular
Purification
Immunogen affinity purified.
Form
Liquid
Function
Enhances caspase-9-mediated apoptosis. Induces NF-kappa- B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS) . Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathway activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN) -derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Recruits NLRP10 to the cell membrane following bacterial infection. .
References & Citations
1. Chamaillard, M., Hashimoto, M., Horie, Y., Masumoto, J., Su, Q., Saab, L., Ogura, Y., Kawasaki, A., Fukase, K., Kusumoto, S., Valvano, M. A., Foster, S. J., Mak, T. W., Nunez, G., Inohara, N. An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid. Nature Immun. 4: 702-707, 2003. 2. da Silva Correia, J., Miranda, Y., Austin-Brown, N., Hsu, J., Mathison, J., Xiang, R., Zhou, H., Li, Q., Han, J., Ulevitch, R. J. Nod1-dependent control of tumor growth. Proc. Nat. Acad. Sci. 103: 1840-1845, 2006. 3. Martinon F, Tschopp J (2006) . NLRs join TLRs as innate sensors of pathogens..Trends Immunol. 26 (8) : 447–54.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
107691 MW
Specificity
No cross reactivity with other proteins.
Applications Notes
6
Gene Name Synonym
Nucleotide-binding oligomerization domain-containing protein 1
Subcellular Location
Cytoplasm. Cell membrane. Apical cell membrane. Basolateral cell membrane. Detected in the cytoplasm and at the cell membrane. Following bacterial infection, localizes to bacterial entry sites in the cell membrane. Recruited to the basolateral and apical membranes in polarized epithelial cells.
Sequence Similarities
Contains 1 CARD domain.
Protein Name
Nucleotide-binding oligomerization domain-containing protein 1
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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