Anti-NR5A2/LRH1 Antibody Picoband®

NR5A2 (nuclear receptor subfamily 5, group A, member 2) also known as liver receptor homolog-1 (LRH-1) is a protein that in humans is encoded by the NR5A2 gene. LRH-1 is a member of the nuclear receptor family of intracellulartranscription factors. LRH-1 plays a critical role in the regulation of development, cholesterol transport, bile acid homeostasis andsteroidogenesis. LRH-1 is important for maintaining pluripotence of stem cells during embryonic development. Liver receptor homolog-1 has been shown to interact with the small heterodimer partner.

Nuclear receptor subfamily 5 group A member 2; Alpha-1-fetoprotein transcription factor; B1-binding factor; hB1F; CYP7A promoter-binding factor; Hepatocytic transcription factor; Liver receptor homolog 1; LRH-1; NR5A2; B1F, CPF, FTF

E.coli-derived human NR5A2 recombinant protein (Position: K44-R237) . Human NR5A2 shares 95% amino acid (aa) sequence identity with both mouse and rat NR5A2.

Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells.

Developmental Biology, Embryogenesis, Embryonic Stem Cells, Epigenetics and Nuclear Signaling, Host-Virus Interaction, Interspecies Interaction, Intracellular, Microbiology, Stem Cells, Surface Molecules, Transcription

Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.

1. Goodwin, B., Jones, S. A., Price, R. R., Watson, M. A., McKee, D. D., Moore, L. B., Galardi, C., Wilson, J. G., Lewis, M. C., Roth, M. E., Maloney, P. R., Willson, T. M., Kliewer, S. A. A regulatory cascade of the nuclear receptors FXR, SHP-1, and LRH-1 represses bile acid biosynthesis. Molec. Cell 6: 517-526, 2000. 2. Gu, P.; Goodwin, B.; Chung, A. C. -K.; Xu, X.; Wheeler, D. A.; Price, R. R.; Galardi, C.; Peng, L.; Latour, A. M.; Koller, B. H.; Gossen, J.; Kliewer, S. A.; Cooney, A. J. (2005) . Orphan Nuclear Receptor LRH-1 is Required to Maintain Oct4 Expression at the Epiblast Stage of Embryonic Development. Molecular and Cellular Biology 25 (9) : 3492–3505.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.