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Anti-Pin1 Antibody Picoband®

Boster Bio Anti-Pin1 Antibody Picoband® catalog # PB9316. Tested in Flow Cytometry, ICC/IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, also called DOD, is an enzyme that in humans is encoded by the PIN1 gene. It is mapped to 19p13.2. The enzyme binds to a subset of proteins and thus plays a role as a post phospho PIN1rol in regulating protein function. Studies have shown that the deregulation of PIN1 may play a pivotal role in various diseases. Notably, the up-regulation of PIN1 may be implicated in certain cancers, and the down-regulation of Pin1 may be implicated in Alzheimer's disease. Inhibitors of PIN1 may have therapeutic implications for cancer and immune disorders. PIN1 activity regulates the outcome of proline-ed kinase (e.g. MAPK, CDK or GSK3) signalling and consequently regulates cell proliferation (in part through control of cyclin D1 levels and stability) and cell survival. PIN1 also has an essential role in maintaining cell proliferation and regulating cyclin D1 function.

Synonyms

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1;5.2.1.8; Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin1; Rotamase Pin1; PIN1

Gene Name

PIN1

Gene ID

5300

UniProt

Q13526

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Pin1 recombinant protein (Position: M1-E163) . Human Pin1 shares 95% amino acid (aa) sequence identity with mouse Pin1.

Clonality

Polyclonal

Tissue Specificity

The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells. .

Applications

Flow Cytometry, ICC/IF, IHC, WB

Field of Research

Alzheimer's Disease, Cell Biology, Cell Cycle, Cell Division, Neurodegenerative Disease, Neurology Process, Neuroscience

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr- Pro) motifs in a subset of proteins, resulting in conformational changes in the proteins (PubMed:21497122, PubMed:22033920) . Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed:16644721) . Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed:15664191) . Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269) . Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed:22608923) . .

References & Citations

1. Liou, Y.-C., Ryo, A., Huang, H.-K., Lu, P.-J., Bronson, R., Fujimori, F., Uchida, T., Hunter, T., Lu, K. P. Loss of Pin1 function in the mouse causes phenotypes resembling cyclin D1-null phenotypes. Proc. Nat. Acad. Sci. 99: 1335-1340, 2002. 2. Lu, KP; Finn G; Lee TH; Nicholson LK (Oct 2007) . Prolyl cis-trans isomerization as a molecular timer.Nat. Chem. Biol. (United States) 3 (10) : 619–29.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

18243 MW

Observed Molecular Weight

18 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1

Subcellular Location

Nucleus . Nucleus speckle . Cytoplasm . Colocalizes with NEK6 in the nucleus (PubMed:16476580) . Mainly localized in the nucleus but phosphorylation at Ser-71 by DAPK1 results in inhibition of its nuclear localization (PubMed:21497122) . .

Sequence Similarities

Contains 1 PpiC domain.

Protein Name

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

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