Anti-Midkine/MDK Antibody Picoband® Fluoro488 Conjugated

Midkine (MK or MDK), also known as neurite growth-promoting factor 2 (NEGF2), is a protein that in humans is encoded by the MDK gene. Midkine is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin (NEGF1, 46% homologous with MK) . It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. Midkine is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. A molecular complex containing receptor-type tyrosine phosphatase zeta (PTPζ ), low density lipoprotein receptor-related protein (LRP1), anaplastic leukemia kinase (ALK) and syndecans is considered to be its receptor.

Midkine; MK; Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; MDK; MK1, NEGF2

E.coli-derived human Midkine recombinant protein (Position: V21-D143) . Human Midkine shares 87% and 89% amino acid (aa) sequence identity with mouse and rat Midkine, respectively.

Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (By similarity) . .

1. Ezquerra, L., Herradon, G., Nguyen, T., Silos-Santiago, I., Deuel, T. F.Midkine, a newly discovered regulator of the renin-angiotensin pathway in mouse aorta: significance of the pleiotrophin/midkine developmental gene family in angiotensin II signaling.Biochem. Biophys. Res. Commun. 333: 636-643, 2005. 2. Inoh, K., Muramatsu, H., Ochiai, K., Torii, S., Muramatsu, T.Midkine, a heparin-binding cytokine, plays key roles in intraperitoneal adhesions.Biochem. Biophys. Res. Commun. 317: 108-113, 2004.