Anti-CD146/MCAM Antibody Picoband® Fluoro647 Conjugated

CD146 (cluster of differentiation 146), also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM, a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. By radiation hybrid analysis, this gene is mapped to chromosome 11q23.3. MCAM has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. MCAM has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency mesenchymal stem cells with greater differentiation potential express higher levels of MCAM on the cell surface.

Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD146; MCAM; MUC18

E.coli-derived human CD146 recombinant protein (Position: H59-A401) . Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146.

Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

Cancer, Cell Adhesion, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Immunology, Invasion/Microenvironment, Signal Transduction, Stem Cells

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. .

1. Bardin, N., Anfosso, F., Masse, J.-M., Cramer, E., Sabatier, F., Le Bivic, A., Sampol, J., Dignat-George, F.Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion.Blood 98: 3677-3684, 2001. 2. Kuske, M. D. A., Johnson, J. P.Assignment of the human melanoma cell adhesion molecule gene (MCAM) to chromosome 11 band q23.3 by radiation hybrid mapping.Cytogenet. Cell Genet. 87: 258 only, 1999. 3. Taira, E., Nagino, T., Taniura, H., Takaha, N., Kim, C.-H., Kuo, C.-H., Li, B.-S., Higuchi, H., Miki, N.Expression and functional analysis of a novel isoform of gicerin, an immunoglobulin superfamily cell adhesion molecule.J. Biol. Chem. 270: 28681-28687, 1995.