Anti-FGF1 Antibody Picoband® Fluoro647 Conjugated

Fibroblast growth factor 1 (acidic), also known as FGF1/ECGF/HBGF1, is a human gene which is mapped to 5q31. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Acidic fibroblast growth factor is derived from beta-endothelial cell growth factor (ECGFB) by posttranslational processing. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor.

Fibroblast growth factor 1; FGF-1; Acidic fibroblast growth factor; aFGF; Endothelial cell growth factor; ECGF; Heparin-binding growth factor 1; HBGF-1; FGF1; FGFA

E.coli-derived human FGF1 recombinant protein (Position: N22-L146) . Human FGF1 shares 97% amino acid (aa) sequence identity with both mouse and rat FGF1.

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. .

1. Le Beau, M. M.; Espinosa, R., III; Neuman, W. L.; Stock, W.; Roulston, D.; Larson, R. A.; Keinanen, M.; Westbrook, C. A. : Cytogenetic and molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases. Proc. Nat. Acad. Sci. 90: 5484-5488, 1993. 2. Mergia, A.; Eddy, R.; Abraham, J. A.; Fiddes, J. C.; Shows, T. B. : The genes for basic and acidic fibroblast growth factors are on different human chromosomes. Biochem. Biophys. Res. Commun. 138: 644-651, 1986. 3. Burgess, W. H.; Mehlman, T.; Marshak, D. R.; Fraser, B. A.; Maciag, T. : Structural evidence that endothelial cell growth factor beta is the precursor of both endothelial cell growth factor alpha and acidic fibroblast growth factor. Proc. Nat. Acad. Sci. 83: 7216-7220, 1986.

Secreted. Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytosol. Nucleus. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu (2+) ions and S100A13. Secreted in a complex with SYT1 (By similarity) . Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59. .