Anti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband®

NHE1, also known as SLC9A1, is an isoform of sodium–hydrogen antiporter that in humans is encoded by the SLC9A1 gene. It is mapped to 1p36.11. NHE1 is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others. The plasma membrane ion exchanger NHE1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. And a structural role for NHE1 in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. NHE1 is a plausible candidate gene for human essential hypertension.

Sodium/hydrogen exchanger 1; APNH; Na (+) /H (+) antiporter, amiloride-sensitive; Na (+) /H (+) exchanger 1; NHE-1; Solute carrier family 9 member 1; SLC9A1; APNH1, NHE1

E.coli-derived human SLC9A1 recombinant protein (Position: H543-Q815) . Human SLC9A1 shares 92% and 93% amino acid (aa) sequences identity with mouse and rat SLC9A1, respectively.

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. .

1. Fliegel L, Dyck JR, Wang H, Fong C, Haworth RS (August 1993) . Cloning and analysis of the human myocardial Na+/H+ exchanger. Mol. Cell. Biochem. 125 (2) : 137–43. 2. Mattei MG, Sardet C, Franchi A, Pouysségur J (1988) . The human amiloride-sensitive Na+/H+ antiporter: localization to chromosome 1 by in situ hybridization. Cytogenet. Cell Genet. 48 (1) : 6–8. 3. Denker, S. P., Huang, D. C., Orlowski, J., Furthmayr, H., Barber, D. L. binding of the Na-H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H (+) translocation. Molec. Cell 6: 1425-1436, 2000. 4. Dudley, C. R. K., Giuffra, L. A., Tippett, P., Kidd, K. K., Reeders, S. T. The Na+/H+ antiporter: a 'melt' polymorphism allows regional mapping to the short arm of chromosome 1. Hum. Genet. 86: 79-83,1990.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell membrane; Multi-pass membrane protein. Colocalizes with CHP1 at the reticulum endoplasmic (By similarity) . Colocalizes with CHP1 and CHP2 at the plasma membrane. .