Anti-HIF1 beta/ARNT Antibody Picoband® Fluoro647 Conjugated
Product Specifications
Background
ARNT is also known as HIF1-beta or HIF1B. This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. It is mapped to 1q21.3. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. ARNT is a structural component of the XRE-binding form of the Ah receptor. It also functions in concert with RelB in a CD30-induced negative feedback mechanism.
Synonyms
Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor, nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta; ARNT; BHLHE2
Gene Name
ARNT
Gene ID
405
UniProt
P27540
Host
Rabbit
Reactivity
Human
Cross Reactivity
No cross-reactivity with other proteins
Immunogen
E.coli-derived human HIF1 beta recombinant protein (Position: V416-E789) . Human HIF1 beta shares 86% and 83% amino acid (aa) sequences identity with mouse and rat HIF1 beta, respectively.
Clonality
Polyclonal
Tissue Specificity
Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. .
Applications
Flow Cytometry
Field of Research
Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Response To Hypoxia, Transcription
Purification
Immunogen affinity purified.
Form
Liquid
Function
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
References & Citations
1. Reyes, H., Reisz-Porszasz, S., Hankinson, O. Identification of the Ah receptor nuclear translocator protein (Arnt) as a component of the DNA binding form of the Ah receptor. Science 256: 1193-1195, 1992. 2. Wright, C. W., Duckett, C. S. The aryl hydrocarbon nuclear translocator alters CD30-mediated NF-kappa-B-dependent transcription. Science 323: 251-255, 2009.
Storage Conditions
At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Calculated Molecular Weight
86636 MW
Specificity
No cross reactivity with other proteins.
Applications Notes
6
Gene Name Synonym
Aryl hydrocarbon receptor nuclear translocator
Subcellular Location
Nucleus.
Sequence Similarities
Contains 1 bHLH (basic helix-loop-helix) domain.
Protein Name
Aryl hydrocarbon receptor nuclear translocator
Isotype
Rabbit IgG
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
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