Anti-AP2 alpha/TFAP2A Antibody Picoband®

TFAP2A, also known as AP-2alpha or BOFS, is a protein that in humans is encoded by the TFAP2A gene. It is mapped to 6p24.3. The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. TFAP2A acts as a sequence-specific DNA-binding transcription factor recognizing and binding to the specific DNA sequence and recruiting transcription machinery. This gene is expressed in neural crest cell lineages with the highest levels of expression corresponding to early neural crest cells, suggesting that TFAP2A plays a role in their differentiation and development.

Transcription factor AP-2-alpha; AP2-alpha; AP-2 transcription factor; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2; TFAP2A; AP2TF, TFAP2

E.coli-derived human AP2 alpha recombinant protein (Position: M1-G166) . Human AP2 alpha shares 98% amino acid (aa) sequence identity with both mouse and rat AP2 alpha.

Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. .

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. .

1. Williams T, Tjian R (April 1991) . Analysis of the DNA-binding and activation properties of the human transcription factor AP-2. Genes & Development 5 (4) : 670–82. 2. Mitchell PJ, Wang C, Tjian R (September 1987) . Positive and negative regulation of transcription in vitro: enhancer-binding protein AP-2 is inhibited by SV40 T antigen. Cell 50 (6) : 847–61. 3. Williams T, Admon A, Lüscher B, Tjian R (December 1988) . Cloning and expression of AP-2, a cell-type-specific transcription factor that activates inducible enhancer elements. Genes & Development 2 (12A) : 1557–69. 4. Le Douarin NM, Ziller C, Couly GF (September 1993) . Patterning of neural crest derivatives in the avian embryo: in vivo and in vitro studies. Developmental Biology 159 (1) : 24–49.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.